Ladies versus Butlers! follows Hino Akiharu, an orphan who was taken in by his uncle's family and grows up determined not to become a burden. He enters Hakureiryou High School, a free boarding school with a mostly female student body, hoping to work there as a butler and earn his own place. Instead, his delinquent, boy-like appearance makes him seem far more intimidating than he really is, and his first days are filled with misunderstandings, awkward encounters, and the challenge of finding his footing in a school that is not prepared for him.
At Hakureiryou, Akiharu unexpectedly reunites with Saikyou Tomomi, the childhood crush he meets again in this unusual setting. Surrounded by social tension and the pressure of fitting in, he has to navigate daily life among classmates who do not know what to make of him. The series mixes school comedy and romantic mishaps around Akiharu's search for acceptance, while the butler-and-ladies premise keeps pushing him into new situations with the girls around him.